Post by account_disabled on Mar 15, 2024 23:20:24 GMT -5
PCR tests have become a vital tool to determine the extent of the Covid- pandemic because they offer the greatest reliability. But how does this test work? Basically, it consists of amplifying a fragment of the patient's genetic material to see if it contains genetic material (RNA) of the SARS-CoV- virus. At the Higher Council for Scientific Research (CSIC), researchers from the Alberto Sols Biomedical Research Institute (IIBM-CSIC-UAM), during seven weeks between May and June, have analyzed , samples from patients from nursing homes and of residences for people with disabilities in the Community of Madrid. “A PCR is a snapshot at a given moment. What we “look at” in a PCR is whether a person has the virus or not at that specific moment. You can be negative when taking the sample and then becoming infected,” explains CSIC researcher Gemma Rodríguez-Tarduchy, head of the Genomics service at the.
The PCR ( Polymerase Chain Reaction ) test is a technique developed in the s by Kary Mullis, who would later win the Nobel Prize. It consists of specifically replicating the genetic material extracted from a patient until millions or billions of copies are obtained; that is, until sufficient quantity is obtained to analyze it and so that the result of that analysis has a high degree of reliability. This amplification capacity AOL Email List makes it a very useful tool not only in biomedical research but also in obtaining a diagnosis, in criminological analysis or in carrying out paleontological studies. Currently, the applications of the PCR technique are innumerable. Antonio Barbachano Becerril, IIBM researcher, introduces a plate with samples into the robot to obtain their genetic material. Vinca Page /CSIC Communication “Many times the problem we encounter when we take a sample is the little genetic material we have to work with. Therefore, what a PCR does is amplify, photocopy the genetic material from an original template. The more photocopies I make, the more of that original mold I have.
Once I have a lot of that mold, I am already able to analyze it,” he adds. Phases of a PCR, from sample collection to diagnosis The PCR test cycle has two main parts: obtaining the sample and performing the analysis. Obtaining is made up of three steps: taking the sample, inactivating the virus and extracting the genetic material. Only later is the PCR analysis technique itself performed. First step: obtain the patient's sample. Healthcare personnel insert a swab through the patient's nasopharyngeal tract until the sample is taken. This swab is inserted into a tube identified with a code that allows traceability of the sample. Furthermore, inside the tube contains a liquid that stabilizes the sample and preserves it. Second step: inactivate the sample . It consists of nullifying the contagious capacity of the virus in a level biological containment laboratory. This step is carried out in collaboration with the Department of Preventive Medicine, Public Health and Microbiology of the Faculty of Medicine of the Autonomous University of Madrid.
The PCR ( Polymerase Chain Reaction ) test is a technique developed in the s by Kary Mullis, who would later win the Nobel Prize. It consists of specifically replicating the genetic material extracted from a patient until millions or billions of copies are obtained; that is, until sufficient quantity is obtained to analyze it and so that the result of that analysis has a high degree of reliability. This amplification capacity AOL Email List makes it a very useful tool not only in biomedical research but also in obtaining a diagnosis, in criminological analysis or in carrying out paleontological studies. Currently, the applications of the PCR technique are innumerable. Antonio Barbachano Becerril, IIBM researcher, introduces a plate with samples into the robot to obtain their genetic material. Vinca Page /CSIC Communication “Many times the problem we encounter when we take a sample is the little genetic material we have to work with. Therefore, what a PCR does is amplify, photocopy the genetic material from an original template. The more photocopies I make, the more of that original mold I have.
Once I have a lot of that mold, I am already able to analyze it,” he adds. Phases of a PCR, from sample collection to diagnosis The PCR test cycle has two main parts: obtaining the sample and performing the analysis. Obtaining is made up of three steps: taking the sample, inactivating the virus and extracting the genetic material. Only later is the PCR analysis technique itself performed. First step: obtain the patient's sample. Healthcare personnel insert a swab through the patient's nasopharyngeal tract until the sample is taken. This swab is inserted into a tube identified with a code that allows traceability of the sample. Furthermore, inside the tube contains a liquid that stabilizes the sample and preserves it. Second step: inactivate the sample . It consists of nullifying the contagious capacity of the virus in a level biological containment laboratory. This step is carried out in collaboration with the Department of Preventive Medicine, Public Health and Microbiology of the Faculty of Medicine of the Autonomous University of Madrid.